منابع مشابه
Albumin turbidity reaction of blood serum.
Turbidity reaction of blood scrum has been developed. The turbidity is formed by serum albumin and comes about when the serum is diluted with MC!LWAINE phosphate-citrate buffer in the range of pH 3.9—4.4. It is significantly enhanced by warming the solution. The temperature must not exceed the point of coagulation. The reaction is suitable as a screening-test of pathological anomalies of serum ...
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There are the dye-binding method, the biuret method, and the turbidimetric method for determining urine protein. Of the turbidimetric methods, a SSA method that is highly sensitive and reliable is used not only to determine the urine protein concentration but also to confirm a false-positive in a test strip and to screen the Bence-Jones proteins from the discrepancy between the test strip and S...
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The interaction of bovine serum albumin ( BSA) and egg albumin with water solublephthalocyanine, cobalt (ΙΙ) 4, 4′ , 4′′, 4′′′- tetrasulfophthalocyanine ( CoTSPc) , has been studiedby the UV- Vis method at pH 7.0 and five different temperatures 20, 25, 30, 35 and 40°C. Theformation constants have been elucidated by using spectrophotometric titration and computerSQUAD program data refinement. Th...
متن کاملProton-transfer reaction dynamics within the human serum albumin protein.
We report on femto- to nanosecond studies of the excited state intermolecular proton transfer (ESPT) reaction of trisodium 8-hydroxypyrene-1,3,6-trisulfonate (pyranine, HPTS) with the human serum albumin (HSA) protein. The formed robust 1:1 complexes (K(eq) = (2.6 ± 0.1) × 10(6) M(-1)) show both photoacid (∼430 nm) and conjugated photobase (∼500 nm) emissions of the caged HPTS in its protonated...
متن کاملA modified method for the quantitative determination of the thymol turbidity reaction of serum.
It was observed by Maclagan that turbidity is produced in a barbital buffer saturated with thymol when specimens of serum from patients with diseases of the liver are added (1, 2). The degree of turbidity apparently varied with the degree of hepatic parenchymal damage. An analysis of the precipitate demonstrated that it contained globulin, phospholipid, cholesterol, and thymol. The mechanism of...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1967
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1967.5.4.187